Stellaris™ fluorescence in situ hybridization (FISH) probes: a powerful tool for mRNA detection
نویسندگان
چکیده
منابع مشابه
StellarisTM fluorescence in situ hybridization (FISH) probes: a powerful tool for mRNA detection
Sensitive enough to detect individual molecules of mRNA, Stellaris FISH probe sets consist of multiple singly labeled oligonucleotides (generally 30–48) designed to hybridize along targeted RNA transcripts. Each discrete transcript is observed as a diffraction-limited spot by conventional fluorescence microscopy. The relatively large number of probes used to detect a single mRNA molecule makes ...
متن کاملDetection of viral RNA by fluorescence in situ hybridization (FISH).
Viruses that infect cells elicit specific changes to normal cell functions which serve to divert energy and resources for viral replication. Many aspects of host cell function are commandeered by viruses, usually by the expression of viral gene products that recruit host cell proteins and machineries. Moreover, viruses engineer specific membrane organelles or tag on to mobile vesicles and motor...
متن کاملFluorescent In Situ Hybridization (FISH) Fluorescent in situ hybridization (FISH) is a fluorescence probe
The importance of cancer biomarkers is now increasingly recognized and acted on by regulatory organizations, healthcare providers (from reimbursement through to practicing clinicians), cancer organizations, research institutes, and charities. For example, the American Joint Committee on Cancer (AJCC) states in the opening of its mission statement, “The purpose and scope of the AJCC is to create...
متن کاملTips and Tricks in Fluorescence In-situ Hybridization (FISH)- based Preimplantation Genetic Diagnosis /Screening (PGD/PGS)
As numerical and structural defects in chromosomes are an inevitable consequence of IVF, Pre-implantation genetic diagnosis and screening (PGD/PGS) methods are used for detecting abnormalities in embryos before implantation to the uterus to increase the successful rate of IVF. Pre-implantation genetic diagnosis and screening approaches can be achieved by different techniques such as NGS, CGH an...
متن کاملFluorescence In Situ Hybridization (FISH) - Application Guide.
Reading a book is also kind of better solution when you have no enough money or time to get your own adventure. This is one of the reasons we show the fluorescence in situ hybridization fish application guide as your friend in spending the time. For more representative collections, this book not only offers it's strategically book resource. It can be a good friend, really good friend with much ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Methods
سال: 2011
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.f.349